on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. 0 × 107) RLU 0. When the user applies a set temperature of 37°C, the top is heated to 37. 5. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. Add to Cart. D. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The excitation and the emission. Multimode microplate readers deliver both performance and economy. The SpectraMax Plus 384 Microplate Reader for. 100 readings per well gives optimal Z’ values for this assay. 7°C and the bottom to 36. For a higher degree of automation, the Automation. It is particularly powerful when monitoring fast kinetic assays in a high. Reader Documentation Version No. 9000-0756. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. D 59 A. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The drawing shows a standard microplate of 15 mm height covered with a lid. 2. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 3) At the bottom of the strip, click on "Show. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. E Models. Published Dec 16, 2020 | Updated Dec 16, 2020. 7. 1) Select the correct plate and the wells that you want to measure. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. Microplate Readers Include the M3, M4, M5, and M5. both cuvette-based and microplate. Perform the following steps to install the drivers: Start > Control Panel > System. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The University of Rouen. 1. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Step 2: Click SoftMax Pro’s Read button. Add to Cart. The fluorescence was measured in the SpectraMax M3 Microplate reader. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. It is typically used to determine the concentration of nucleic acids and proteins. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. 25 to 3. Other technologies such as imaging. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. . Dose-dependent enzyme essayVisualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). Sale price $9,995. Highly selective for dsDNA over ssDNA or RNA. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. The Cut-off filter for this pair is showing 530nm in this machine. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). flexibility of monochromator-based systems. Package performance tests. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Description 1. Microplate Reader Shelf. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. was 1. 8 Customer Reviews. Add to Cart. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Appendix. A). 9000-0756. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Suggested plates for best data: Fluorescence-top read; Solid Black flat-bottom for Excitations above 350 nm;. 1. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Add to Cart. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The OliGreen assay from Molecular Probes, when run on a Gemini XS or Gemini EM fluorescence microplate reader with SoftMax Pro software, is a quick, sensitive detection method for single-stranded DNA. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. Enables accurate fluorescence data with every run. Search: Submit search Search filters Search This site Search Cornell. Molecular Devices SpectraMax M2 Multimode Microplate Reader w/ Laptop & Software. . The fluorescence intensity of MitoTracker® Red CM-H2XRos was measured with a fluorescence plate reader at excitation and. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. spr SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. 25 to 3. Endpoint, kinetic, spectrum. 0 out of 5 $ 3,895. It may be named "Pothos". Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Filters: Excitation and emission slides for 4 filters each. 3 kg. The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. The password is. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. 8 foot) 4400-0002 Power cord, 1 meter (3. )Molecular Devices SpectraMax M2 Microplate Reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. 5%in96-and384-wellmicroplates. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. S. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. 1. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. NanoQuant plate. Standard. Update Driver. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Usage/Application: Lab. Similar settings will work for other SpectraMax readers with fluorescence detection. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. With exceptional signal-to-noise ratio and a lower detection limit. Double-click the unknown device with the yellow warning icon. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. For a higher . Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 303 unable to cal dark current. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The GloMax® line includes high-performance plate readers with a range of detection options. Page 28 Chapter 5: Read Modes and Read Types 1. Brand: MOLECULAR DEVICES. Refurbished. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. 3: The cuvette chamber. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. SpectraMax Quant dsDNA Assay Kits. The SpectraMax i3 Multi-Mode Microplate Reader and the SpectraMax MiniMax 300 Imaging Cytometer enable both ways of assessing transfection conditions for GFPbased reporter assays. 2. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. The chamber. Multimode Reader Comparison Chart. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. . The SpectraMax 190 reader can be integrated with Molecular . Published Dec 16, 2020 | Updated Dec 16, 2020. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. When a feature or capability applies to only certain readers, this exception is noted. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 7°C (gradient heating design). The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Offer. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. 1. On microplate readers,. 1. 5%in96-and384-wellmicroplates. Figure 6: Transcreener ADP2 FI assay principle. Also refer to this Multimode Reader Comparison Chart. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. The pre-configured protocols can be selected for each assay and. 25 to 3. 5,112,134 5,766,875. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. ~ Bio-Tek ELx808 Microplate Reader $2,795. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. 0 out of 5 $ 9,995 $ 9,495. 0) OD (0. Category 2 and 3 microplate readers use gradient heating as well. Tecan Infinite F200 Fluorescence Microplate Reader. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. SpectraMax Spectrofluorometers. This can increase throughput and efficiency, thus saving time and space in the lab. In general, reading fluorescence from the top is more sensitive than reading from the bottom. D 47 7. Skip to main content. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Avoid losing high signal. 00. Results were graphed in SoftMax Pro Software using a 4-parameter curve fit. Uses dual. I am using the filter pair of 480/535 and trying to measure fluorescence. Molecular devices SpectraMax M5 Pdf User Manuals. We therefore gathered salient. Appendix A. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2 only) SpectraMax M-series Microplate readers. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. Wide range of applications. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fluorescence Photometric Performance (M2) Dual monochromators: 1 nm increments: EX: 250-850 nm: EM: 250-850 nm: Bandwidth (EX, EM). They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. spr SpectraMax Plus 384 SpectraTest ABS2 v1. 25 to 3. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. 5,112,134 5,766,875. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. Select the lab user profile. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 0077 OD 0. . Biotek Elx800 Microplate Reader. Single-Label Plate Reader: SpectraMax Plus 384 (Molecular Devices). View online or download Molecular devices SpectraMax M3 User Manual. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. A microplate reader asPython 3. 0 × 103 to 2. Last lab had ours for years and never had a problem with it. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The cuvette must be in place when you read the plate. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. Three kits covering a wide range of dsDNA concentrations. Figure 6: Transcreener ADP2 FI assay principle. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Microplate Readers. patent nos. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 3 foot) See. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. The Spectr. Appendix A. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 10. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. SpectraMax M2 and M2e Multi-Mode Microplate Readers User Guide Molecular Devices SpectraMax M2 and M2e Multi-Mode Microplate Readers. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Our absorbance plate readers feature our PathCheck Sensor. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers are as Fluorescence Validation Test Plates provide validation of the system’s optical characteristics. free tracer decreases, resulting in an overall increase in RFU values. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Gemini XPS Fluorescence Microplate Reader . Perkin Elmer 1420-2560 Victor X Series Plate Reader. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Transcreener ® ADP. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Add to Cart. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. 0077 OD 0. The dual monochromator optics allow the. SpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. MPN: 50505. S. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Enter wavelength settings as shown below: 4. 5). 53 - 60 kg, (117-132 lb), depending on configuration. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 0 out of 5 $ 2,999-20%. Wavelength optimization. 1. The analysis capabilities of SoftMax Pro Software provide quantitation in an easy-to-read, user-customizable report format. When connecting for the first time to either the SpectraMax L reader or StakMax system. BMG LABTECH. Sale. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual. When a. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Microplate Reader Shelf. Bioz Stars score: 86/100, based on 1 PubMed citations. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Published Dec 16, 2020 | Updated Dec 16, 2020. Figure 2. Align the two slots on the bottle holder with the two pegs on the rail. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. $ 2,499. Fixes: General:SpectraMax M2 and M2e Multi-Mode. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. min at 37°C. 2. FI assay. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. SoftMax Pro. S. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Add to Cart. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. In the M2, M5 and Plus. Microplate Reader, Multimode. 0) OD (500 to 1100) RFU (5. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. D 47 7. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. SpectraMax Paradigm Multi-Mode Safety Guide The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. ₹ 1,80,000 Get Latest Price. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. for the plate. Offer Ends In: 45 Days 06: 20: 11. We use Spectramax M5 microplate reader in our lab. A . 1 x 38. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Cells in each image are identified by SoftMax Pro Software and cell-by-cell fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. fluorescence). We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. Refurbished. Fluorescent Polarization: Solid black plates, as above. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. B. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). begin reading microplates with seven mouse clicks. Microplate Readers. Downloads. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Microplate Formats: 6, 12, 24, 48, 96, 384.